GLYOXAL SOLUTION FOR MOLECULAR BIOLOGY BIOREAGENT ~40% IN H2O (~8.8 M) (C005B-006296)

General description

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

Application

Suitable for

• nucleic acid denaturation
• binding of DNA/RNA to nitrocellulose membrane

Other Notes

This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.

May precipitate on storage; redissolve at 50-60 Â°C.

Caution

May precipitate on storage; redissolve at 50-60 °C.

Principle

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

Molecular Weight: 58.04. Empirical Formula: C₂H₂O₂. linearFormula: OHCCHO. grade: for molecular biology. Quality Level: 100. product line: BioReagent. form: liquid. concentration: ~40% in H₂O (~8.8 M). impurities: DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected. refractive index: n20/D 1.409. anion traces: chloride (Cl^-): ≤. 100 . mg/kg, sulfate (SO₄^2-): ≤. 100 . mg/kg. cation traces: Ca: ≤. 5 . mg/kg, Cd: ≤. 5 . mg/kg, Co: ≤. 5 . mg/kg, Cr: ≤. 5 . mg/kg, Cu: ≤. 5 . mg/kg, Fe: ≤. 5 . mg/kg, K: ≤. 5 . mg/kg, Mg: ≤. 5 . mg/kg, Mn: ≤. 5 . mg/kg, Na: ≤. 300 . mg/kg, Ni: ≤. 5 . mg/kg, Pb: ≤. 5 . mg/kg, Zn: ≤. 5 . mg/kg. storage temp.: 2-8°C. SMILES string: [H]C(: O)C([H]): O. InChI: 1S/C2H2O2/c3-1-2-4/h1-2H. InChI key: LEQAOMBKQFMDFZ-UHFFFAOYSA-N. Pictograms: GHS07,GHS08. Signal Word: Warning. Hazard Statements: H315 - H317 - H319 - H332 - H341. Precautionary Statements: P201 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313. Hazard Classifications: Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.