ETHIDIUM BROMIDE SOLUTION BIOREAGENT FOR MOLECULAR BIOLOGY 10 MG/ML IN H2O (C005B-012552)

Application

Ethidium bromide solution has been used:

• as a stain to visualize U937 cells to assess cell viability
• to detect polymerase chain reaction products
• in agarose gel electrophoresis based gel retardation assay

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Molecular Weight: 394.31. Empirical Formula: C₂₁H₂₀BrN₃. grade: for molecular biology. Quality Level: 200. product line: BioReagent. concentration: 10 . mg/mL in H₂O. technique(s): electrophoresis: suitable. suitability: suitable for gel electrophoresis. SMILES string: [Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14. InChI: 1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14. /h3-13,23H,2,22H2,1H3. 1H. InChI key: ZMMJGEGLRURXTF-UHFFFAOYSA-N. Pictograms: GHS06,GHS08. Signal Word: Danger. Hazard Statements: H331 - H341. Precautionary Statements: P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313. Hazard Classifications: Acute Tox. 3 Inhalation - Muta. 2. Storage Class Code: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects. WGK: nwg. Flash Point(F): Not applicable. Flash Point(C): Not applicable.