INTERLEUKIN-3 FROM MOUSE IL-3 RECOMBINANT EXPRESSED IN E. COLI LYOPHILIZED POWDER SUITABLE FOR CELL CULTURE (C005B-223427)

General description

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

Application

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

biological source: mouse. Quality Level: 200. recombinant: expressed in E. coli. Assay: ≥. 98% (SDS-PAGE). form: lyophilized powder. potency: 0.01-0.1 . ng/mL ED₅₀/EC₅₀. quality: endotoxin tested. mol wt: 15.1 . kDa. packaging: pkg of 10 and 50 . μ. g. technique(s): cell culture | mammalian: suitable. impurities: ≤. 1.000 . EU/μ. g. color: white to yellow. UniProt accession no.: P01586. storage temp.: −. 20°C. Gene Information: mouse ... Il3(16187). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).