INTERLEUKIN-3 HUMAN IL-3 RECOMBINANT EXPRESSED IN E. COLI LYOPHILIZED POWDER SUITABLE FOR CELL CULTURE (C005B-223349)

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized with no additives. Sterile filtered through a 0.2 μm filter.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

biological source: human. Quality Level: 200. recombinant: expressed in E. coli. Assay: ≥. 97% (HPLC and SDS-PAGE). form: lyophilized powder. potency: ≤. 1.0 . ng/mL ED₅₀/EC₅₀. quality: endotoxin tested. mol wt: 15 . kDa. packaging: pkg of 10 and 50 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: ≤. 0.1 . EU/μ. g. UniProt accession no.: P08700. storage temp.: −. 20°C. Gene Information: human ... IL3(3562). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.