INTERLEUKIN-4 FROM MOUSE IL-4 RECOMBINANT EXPRESSED IN E. COLI LYOPHILIZED POWDER SUITABLE FOR CELL CULTURE

Application

Interleukin-4 from mouse has been used:

• as a component of cell culture media for class switch recombination assay
• to study its therapeutic benefits for mucosal healing
• as a pioglitazone or new peroxisome proliferator-activated receptor agonist (GQ-11)

Biochem/physiol Actions

Interleukin-4 (IL-4) is a lymphokine with profound effects on the growth and differentiation of immunologically competent cells. IL-4 is also known as B cell stimulatory factor-1 (BSF-1), T cell growth factor-2 (TCGF-2) and mast-cell growth factor-2 (MCGF-2). Inhibits VEGF-induced and FGF-Basic-induced angiogenesis. IL-4 is a complex glycoprotein released by a subset of activated T cells. Treatment of IL-4 with specific glycosidases yields an active polypeptide. Human and mouse IL-4 share 50% amino acid sequence homology, but their biological actions are species-specific.

Physical form

Lyophilized, 0.2 μm filtered, with no additives.

Analysis Note

The proliferative activity is tested in culture using mouse HT-2 cells.

biological source: mouse. recombinant: expressed in E. coli. Assay: ≥. 98% (SDS-PAGE). form: lyophilized powder. potency: 0.1-2.0 . ng/mL ED₅₀/EC₅₀. quality: endotoxin tested. mol wt: ~13.5 . kDa. packaging: pkg of 5 . μ. g. technique(s): cell culture | mammalian: suitable. impurities: <. 0.1 . EU/μ. g. UniProt accession no.: P07750. storage temp.: −. 20°C. Gene Information: mouse ... Il4(16189). Storage Class Code: 11 - Combustible Solids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).