INTERLEUKIN-21 RECOMBINANT MOUSE

Biochem/physiol Actions

IL-21 (interleukin-21) belongs to the IL-2 cytokine family. It is secreted by CD4⁺ T, natural killer (NK) and Th17 cells. IL-21 associates with IL-21 receptor and participates in B-cell proliferation, differentiation as well as apoptosis. It also promotes the cytotoxic activity of CD8⁺ T cells and natural killer cells.IL-21 plays a crucial role in the differentiation of follicular helper T cells. It also exhibits antitumor activity. It is also involved in the activation of JAK-STAT (Janus kinase - signal transducer and activator of transcription), PI3K (phosphatidylinositol 3-kinase) and MAPK (mitogen activated protein kinase) pathways.

IL-21 and its receptor have important roles in T and B cell proliferation and natural killer cell maturation. Human and mouse IL-21 share 57% amino acid identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to induce proliferation of human N1186 T cells.

biological source: mouse. recombinant: expressed in E. coli. Assay: ≥. 97% (SDS-PAGE). form: lyophilized powder. potency: 40-120 . ng/mL ED₅₀. mol wt: predicted mol wt ~14.4 . kDa. packaging: pkg of 10 . μ. g. storage condition: avoid repeated freeze/thaw cycles. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. UniProt accession no.: Q9ES17. storage temp.: −. 20°C. Gene Information: mouse ... Il21(60505). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).