INTERLEUKIN 12 MOUSE RECOMBINANT

Application

Interleukin-12 (IL-12) from mouse has been used to study the effect of IL-12 on Th1 (type 1 T helper) immunity in Mongolian gerbils (Meriones unguiculatus) infected with Sporothrix schenckii. It has been used as a standard in ELISA (enzyme-linked immunosorbent assay) cytokine assay.

Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

biological source: mouse. Quality Level: 100. recombinant: expressed in baculovirus infected Sf21 cells. Assay: ≥. 97% (SDS-PAGE). form: lyophilized powder. potency: 0.01-.1 . ng/mL ED₅₀. mol wt: glycoprotein 70 . kDa. packaging: pkg of 10 . μ. g. technique(s): cell culture | mammalian: suitable. impurities: endotoxin, tested. UniProt accession no.: P43431, P43432. storage temp.: −. 20°C. Gene Information: mouse ... Il12a(16159), Il12b(16160). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).