SODIUM CHOLATE HYDRATE BIOREAGENT SUITABLE FOR CELL CULTURE (C005B-024663)

General description

Cholate is a primary bile acid produced from cholesterol in the liver. It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).

Application

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system,
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor

Biochem/physiol Actions

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.

Molecular Weight: 430.55 (anhydrous basis). Empirical Formula: C₂₄H₃₉NaO₅ · xH₂O. biological source: bovine bile, ovine bile. Quality Level: 200. description: anionic. product line: BioReagent. Assay: ≥. 99.0% (HPLC). form: powder. mol wt: micellar avg mol wt 900-1300. aggregation number: 2-3. technique(s): cell culture | mammalian: suitable. CMC: 9-15 . mM (20-25°C). solubility: water: 100 . mg/mL, clear, colorless to light yellow. HLB: 18. SMILES string: O.[Na+].C[C@H](CCC([O-]): O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C. InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26. . /h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29). . 1H2/q. +1. /p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-. . /m1../s1. InChI key: MUVVIYFKOVLQHL-RCVKHMDESA-M. Hazard Statements: H412. Precautionary Statements: P273 - P501. Hazard Classifications: Aquatic Chronic 3. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).