COENZYME A SODIUM SALT HYDRATE BIOREAGENT SUITABLE FOR CELL CULTURE (C005B-048608)

Application

Coenzyme A sodium salt hydrate has been used:

• in the branch chain aminotransferase enzymatic assay and protein determination
• in the assay for halogenation of pyrrolyl-S-Bmp1 (an acyl carrier protein (ACP)-thioesterase (TE) di-domain protein) by Bmp2
• as an extracellular coenzyme A (CoA) in in vitro and in vivo experiments to study alternative routes by which cells and organisms can obtain CoA

Biochem/physiol Actions

Coenzyme A (CoA) is a ubiquitous cofactor that plays an important role in many metabolic pathways, mainly as acyl-CoA. It is essential for the tricarboxylic acid cycle, and the synthesis and oxidation of fatty acids. CoA facilitates acyl group transfers, especially in fatty acid and lipid metabolism reactions. It is associated with neurodegeneration, autophagy, protein acetylation, and signal transduction.

Quality

Enzymatically assayed using phosphotransacetylase. Approx. 95% CoA-SH

Molecular Weight: 767.53 (anhydrous free acid basis). Empirical Formula: C₂₁H₃₆N₇O₁₆P₃S · xNa⁺ · yH₂O. biological source: yeast. Quality Level: 200. description: cofactor for acyl transfer. product line: BioReagent. Assay: ≥. 85% (HPLC). form: powder. technique(s): cell culture | mammalian: suitable. solubility: water: 50 . mg/mL, clear, colorless to faintly yellow. web metadata keyword.default: acyl group transport, fatty acid transport. storage temp.: −. 20°C. SMILES string: [Na+].CC(C)(COP(O)(: O)OP(O)(: O)OC[C@H]1O[C@H]([C@H](O)[C@@H]1OP(O)([O-]): O)n2cnc3c(N)ncnc23)C(O)C(: O)NCCC(: O)NCCS. InChI key: SYTRWOCXZXQBPW-CLVRNSBASA-M. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).