Recombinant EBOV GPDTM (mammalian), 100ug

Description: Mature, recombinant, HA-tagged Ebolavirus Glycoprotein minus the transmembrane domain (EBOV rGPdTM) is supplied as purified protein. EBOV rGPdTM is produced in mammalian cells and is purified by FPLC. Size: 100 µg of protein is supplied in PBS at a concentration of 1.008 mg/mL. The theoretical molecular weight of the protein is ~80-120 kDa including the HA-tag, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms. Relevance: Recombinant glycoprotein provides a means for antibody development, control protein for testing, and a tool to enhance research. Western Blot: Quality control testing demonstrates strong detection of GP null under reduced conditions down to 50 ng when using IBT’s monoclonal antibody 4F3 (cat 0201-020) at 0.5 ug/mL. Storage: 2-3 weeks -20°C, -80°C long term Intended for research use only, not for human, therapeutic, or diagnostic applications.

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