Recombinant BDBV GPDTM, 100ug

Description: Mature, recombinant, His-tagged Bundibugyo glycoprotein minus the transmembrane domain (BEBOV rGPdTM) is supplied as purified protein. BEBOV rGPdTM is produced in Sf9 insect cells using baculovirus for expression and is purified by column chromatography. Size: 100 μg of protein is supplied in PBS (supplemented with 10% glycerol, arginine and glutamic acid) at a concentration of 1.59 mg/mL. The theoretical molecular weight of the protein is ~73 kDa including the His-tag, without glycosylation. Because of the glycosylated nature of this protein, migration in an SDS-PAGE gel results in a broad band. Relevance: Recombinant BEBOV glycoprotein provides a means as a control protein for immunoassays and a tool to enhance Filovirus research. Western Blot: Quality control testing demonstrates strong detection of GP null and GP1 under reduced conditions when using IBT's Rabbit anti-BEBOV GP polyclonal antibody (Cat #: 0304-001). Storage: 2-3 weeks at -20°C, -80°C long term Intended for research use only, not for human, therapeutic, or diagnostic applications.