POLY(2'-DEOXYINOSINIC-2'-DEOXYCYTIDYLICA

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

grade: for molecular biology. Quality Level: 100. form: powder. quality: lyophilized. mol wt: M_r ~2.5 ×. 10⁶ (broad molecular weight distribution), M_r ~2500000. packaging: ampule of ≥. 10 . units A₂₆₀. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).