N-ACETYL-ASP-GLU-VAL-ASP-P-NITROANILIDE

Amino Acid Sequence

NAc-Asp-Glu-Val-Asp-pNA

Application

N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide has been used as a chromogenic substrates for determining caspase-3 and caspase-8 activities in liver tissue homogenates and caspase 3 activity in aortic vascular smooth muscle cells and neuroblastoma cell lines.

Biochem/physiol Actions

Colorimetric substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase.

Molecular Weight: 638.58. Empirical Formula: C₂₆H₃₄N₆O₁₃. Quality Level: 100. Assay: ≥. 97% (HPLC). form: powder. color: white. storage temp.: −. 20°C. SMILES string: CC(C)[C@H](NC(: O)[C@H](CCC(O): O)NC(: O)[C@H](CC(O): O)NC(C): O)C(: O)N[C@@H](CC(O): O)C(: O)Nc1ccc(cc1)[N+]([O-]): O. InChI: 1S/C26H34N6O13/c1-12(2)22(26(43)30-18(11-21(38)39)24(41)28-14-4-6-15(7-5-14)32(44)45)31-23(40)16(8-9-19(34)35)29-25(42)17(10-20(36)37)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-3H3,(H,27,33)(H,28,41)(H,29,42)(H,30,43)(H,31,40)(H,34,35)(H,36,37)(H,38,39)/t16-,17-,18-,22-/m0/s1. InChI key: GGXRLUDNGFFUKI-ORGXJRBJSA-N. Gene Information: human ... CASP3(836). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).