MACROPHAGE COLONY-STIMULATING FACTOR HUMAN M-CSF RECOMBINANT EXPRESSED IN HEK 293 CELLS HUMANKINE(R) SUITABLE FOR CELL CULTURE

General description

M-CSF is encoded by the gene mapped to human chromosome 1p13.3. It is a glycosylated polypeptide, which exists as a homodimer. M-CSF is produced by macrophage, fibroblasts, lymphocyte and endothelial cells.

Biochem/physiol Actions

Macrophage CSF (M-CSF) as a growth factor, stimulates the proliferation and differentiation of monocyte/macrophage-lineage cells. It also boosts antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages. In addition, human M-CSF inhibits bone resorption by osteoclasts. It might also play a crucial role in atherosclerosis. Overexpression of the gene has been observed in Paget′s disease of bone (PDB).

Physical form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells (mouse myeloid Leukemia indicator cell line).

Citation

1. Mazur, E.M., and Cohen, J.L., Clin. Pharmacol. Ther., 46, 250 (1989).
2. Morstyn, G., and Burgess, A.W., Cancer Res., 48, 5624 (1988).
3. Metcalf, D., Blood, 67, 257 (1986).
4. Mufson, R.A., et al., Cell. Immunol., 119, 182 (1989).
5. Hattersley, G., et al., J. Cell Physiol., 137, 199 (1988).

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

biological source: human. Quality Level: 100. recombinant: expressed in HEK 293 cells. Assay: ≥. 95% (SDS-PAGE). form: lyophilized powder. potency: ≤. 4 . ng/mL EC₅₀. quality: endotoxin tested. mol wt: dimer 35-40 . kDa (glycosylated). packaging: pkg of 10 . μ. g. manufacturer/tradename: HumanZyme. technique(s): cell culture | mammalian: suitable. UniProt accession no.: P09603. storage temp.: −. 20°C. Gene Information: human ... CSF1(1435). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.