INTERLEUKIN-2 FROM MOUSE IL-2 RECOMBINANT EXPRESSED IN E. COLI LYOPHILIZED POWDER SUITABLE FOR CELL CULTURE (C005B-223316)

General description

IL-2 (interleukin-2) codes for a pro-inflammatory cytokine. IL-2 is mostly expressed in T helper cells.

Application

Interleukin-2 from mouse has been used to induce sepsis in rat model. It has also been used in dendritic epidermal T cell culture.

Biochem/physiol Actions

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of natural killer cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. IL-2 expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 along with endothelial white blood cells of skeletal muscles. IL-2 regulation is controlled by c-rel protein. IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Analysis Note

Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED₅₀ : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 10⁶ units/mg)

biological source: mouse. Quality Level: 200. recombinant: expressed in E. coli. Assay: ≥. 97% (SDS-PAGE). form: lyophilized powder. potency: ≤. 0.2 . ng/mL ED₅₀. quality: endotoxin tested. mol wt: 17.2 . kDa. packaging: pkg of 5X20 . μ. g, pkg of 20 . μ. g. technique(s): cell culture | mammalian: suitable. impurities: ≤. 1.000 . EU/μ. g. color: white. web metadata keyword.default: TCGF. UniProt accession no.: P04351. storage temp.: −. 20°C. Gene Information: mouse ... Il2(16183). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).