INTERLEUKIN 3 MOUSE (C005B-306179)

General description

IL-3 (interleukin 3) is primarily produced by activated CD4⁺ T cells and mast cells, and is a multi-lineage hematopoietic growth factor and immunoregulatory cytokine.
Recombinant murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues.

Biochem/physiol Actions

IL-3 (interleukin 3) plays a key role in the differentiation of murine hematopoietic progenitor cells and the activation and survival of mature myeloid cells. This protein was originally termed as multicolony-stimulating factor (multi-CSF) because of its capability to induce the differentiation of a wide variety of hematopoietic progenitor cells from bone marrow, such as mast cells, basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes. IL-3 promotes mast cell growth, differentiation, and mediator release, hence, suggesting a role in allergic inflammation. In mice, it is involved in suppressing protective immunity against Plasmodium berghei NK651, and prevents the development of splenomegaly, anemia, and erythropoiesis. It functions as a regulator of osteoclastogenesis, where it play a dual role- it facilitate the development of osteoclast progenitors but suppresses the osteoclastogenic process.

Sequence

MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC

Physical form

Lyophilized from 0.1% TFA.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.