HYDROCORTISONE GAMMA-IRRADIATED CELL CULTURE TESTED (C005B-044934)

General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Application

Hydrocortisone is used for the following applications:

• Used in myogenic induction and identification
• Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
• Used in adipogenic differentiation (one of the components in adipogenic medium)
• Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
• The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
• Used in differentiation assays of melanoma spheroid cells

Biochem/physiol Actions

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na²⁺ retention potency.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

Molecular Weight: 362.46. Empirical Formula: C₂₁H₃₀O₅. biological source: synthetic (organic). Quality Level: 200. sterility: γ. -irradiated. product line: BioXtra. Assay: ≥. 80% (HPLC). form: powder. technique(s): cell culture | mammalian: suitable. mp: 211-214 . °C (lit.). solubility: ethanol: 1 . mg/mL, clear. web metadata keyword.default: hormone solution, adherent cell culture enhancer, epithelial cell culture enhancer, endotehlial cell culture enhancer. shipped in: ambient. storage temp.: room temp. SMILES string: [H][C@@]12CCC3: CC(: O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(: O)CO. InChI: 1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1. InChI key: JYGXADMDTFJGBT-VWUMJDOOSA-N. Gene Information: human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413). Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H360Df - H373. Precautionary Statements: P202 - P260 - P280 - P308 + P313 - P405 - P501. Hazard Classifications: Repr. 1A - STOT RE 2. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges.