AZASERINE (50X) GAMMA-IRRADIATED CELL CULTURE TESTED (C005B-009749)

General description

Chemical structure: amino acid derivatives

Application

For use in hybridoma cell culture applications as a myeloma selection agent. Used to study inhibition of formylglycinamide ribonucleotide amidotransferase and PRPP amidotransferase.

Biochem/physiol Actions

Azaserine is an antibiotic, generated by Streptomyces fragilis. It is considered as a potential mutation inducing agent. It is known to promote pancreatic cancer and induces adenoma and carcinoma in vivo and in vitro. Azaserine also stimulates kidney and liver tumors in rats.

Reconstitution

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 5.7 μM azaserine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Molecular Weight: 173.13. Empirical Formula: C₅H₇N₃O₄. grade: Hybri-Max^™. . Quality Level: 200. sterility: γ. -irradiated. product line: BioXtra. form: lyophilized powder. technique(s): cell culture | hybridoma: suitable. impurities: endotoxin, tested. web metadata keyword.default: Sigma-Aldrich A1164, Azaserine Hybri-Max^™. ,γ. -irradiated, 50x, lyophilized powder, BioXtra, suitable for hybridoma, O-Diazoacetyl-L-serine, glutamine analogue, GLN, IMP biosynthesis inhibitor, inositol monophosphate biosynthesis inhibitor. antibiotic activity spectrum: fungi. Mode of action: enzyme | inhibits. storage temp.: −. 20°C. SMILES string: N[C@@H](COC(: O)C: [N+]: [N-])C(O): O. InChI: 1S/C5H7N3O4/c6-3(5(10)11)2-12-4(9)1-8-7/h1,3H,2,6H2,(H,10,11)/t3-/m0/s1. InChI key: MZZGOOYMKKIOOX-VKHMYHEASA-N. Pictograms: GHS06,GHS08. Signal Word: Danger. Hazard Statements: H301 - H351. Precautionary Statements: P201 - P301 + P310 + P330. Hazard Classifications: Acute Tox. 3 Oral - Carc. 2. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges.