HISTONE H3.1 HUMAN RECOMBINANT EXPRESSED IN <(><<)>I>ESCHERICHIA COLI <(><<)>/I>

General description

Histone H3.1 is a histone H3 variant. It differs from H3.2 with residue 96 being a cysteine instead of serine. H3.1 is a replication-dependent histone and is a component of nucleosomes during the S-phase.

Application

Histone H3.1 human has been used in in vitro Schizosaccharomyces pombe Set7 histone methyltransferase assay for matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) studies.

Biochem/physiol Actions

Histone H3.1 human (H3.1) undergoes a majority of post-translational modification especially acetylation and demethylation in lysine 14 and 9 respectively. During differentiation, the levels of H3.1 transcripts decrease as cell division slows down. The incorporation of H3.1 into chromatin is mediated by a chaperone, chromatin assembly factor (CAF-1). A transversion mutation in the H3.1 may be implicated in glioma.

Physical form

Supplied as 1 mg/mL in 20 mM NaPO₄, pH 7.0, 0.3 M NaCl, 1 mM EDTA, and 1 mM DTT.

biological source: human. Quality Level: 200. recombinant: expressed in E. coli. form: liquid. concentration: 1 . mg/mL. technique(s): MALDI-TOF: suitable. UniProt accession no.: P68431. shipped in: wet ice. storage temp.: −. 20°C. Gene Information: human ... H3C1(8350). Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H319. Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313. Hazard Classifications: Eye Irrit. 2. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.