HISTONE H2A HUMAN RECOMBINANT EXPRESSED IN <(><<)>I>ESCHERICHIA COLI <(><<)>/I>

General description

Histones are highly basic chromatin proteins. They tightly pack and organize eukaryotic DNA to form nucleosomes. A nucleosome comprises an octameric core histone made of a tetramer of histones H3 and H4 with two dimers of histones H2A and H2B. The 147 DNA base pairs are wrapped around the histone complex. H2A consists of a C-terminal tail extending out of the nucleosome core particle.

Application

Histone H2A human has been used as a neutrophil extracellular trap (NET) to study its effects on the membrane integrity and viability of macrophages (MFS) and dendritic cells (DCS). It has also been used in far-western blot analysis for protein identification.

Biochem/physiol Actions

Histones modulate the accessibility of DNA during transcription, DNA replication, DNA repair, and recombination. N and C terminal tails undergo post-translational modifications (PTMs) altering DNA wrapping and chromatin regulation.

Physical form

Supplied in 20 mM Na₃PO₄, pH 7.0, 0.3 M NaCl and 1 mM EDTA

biological source: human. Quality Level: 200. recombinant: expressed in E. coli. form: liquid. concentration: 1 . mg/mL. UniProt accession no.: Q16777. application(s): cell analysis. shipped in: wet ice. storage temp.: −. 20°C. Gene Information: human ... H2AC20(8338). Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H350. Precautionary Statements: P201 - P308 + P313. Hazard Classifications: Carc. 1B. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.