GLY-PRO-ARG-PRO =95% (HPLC) (C005B-056845)

Amino Acid Sequence

Gly-Pro-Arg-Pro

General description

Gly-Pro-Arg-Pro is a peptide that mimics the N-terminal Gly-Pro-Arg region in the α chain of fibrin protein.

Application

Gly-Pro-Arg-Pro (GPRP) has been used for the formation of crystal composed of GPRP and the 30kDa C-terminal fragment from the γ chain of fibrinogen (rFbgγC30), cloned and expressed in Pichia pastoris.

Biochem/physiol Actions

Gly-Pro-Arg-Pro (GPRP) is capable of increasing free thrombin. Thus it can be utilized to make thrombin-degranulated platelets, which helps in studying platelet reactions.

Gly-Pro-Arg-Pro peptide suppresses the early steps of fibrin polymerization. The amide derivative of this peptide prevents the degradation of fibrinogen and fragment D by plasmin, even in the presence of EGTA (ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid).

Inhibits fibrin polymerization.

Molecular Weight: 425.48. Empirical Formula: C₁₈H₃₁N₇O₅. Quality Level: 200. Assay: ≥. 95% (HPLC). UniProt accession no.: P02751, P04937, P11276. storage temp.: −. 20°C. SMILES string: NCC(: O)N1CCC[C@H]1C(: O)N[C@@H](CCCNC(N): N)C(: O)N2CCC[C@H]2C(O): O. InChI: 1S/C18H31N7O5/c19-10-14(26)24-8-2-5-12(24)15(27)23-11(4-1-7-22-18(20)21)16(28)25-9-3-6-13(25)17(29)30/h11-13H,1-10,19H2,(H,23,27)(H,29,30)(H4,20,21,22)/t11-,12-,13-/m0/s1. InChI key: WXPZDDCNKXMOMC-AVGNSLFASA-N. Gene Information: human ... FN1(2335)
mouse ... FN1(14268)
rat ... FN1(25661). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).