GLUCOSE-6-PHOSPHATE DEHYDROGENASE KIT

General description

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia.

Features and Benefits

• Linear detection range (20 μL sample): 0.2 to 100 U/L for 15 minute reaction.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism in plasma, serum, tissue and culture media, etc.

Principle

The non-radioactive, colorimetric Glucose-6-Phosphate Dehydrogenase Assay Kit is based on the reduction of tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.