DMT-2'F-DG(IB) AMIDITE 100G SINGLE (C005B-018126)

General description

DMT-2′Fluoro-dG(ib) Phosphoramidite is a 2′Fluoro phosphoramidite that is used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.

Its key features include,

• Can be employed together with DNA or RNA phosphoramidites
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA for 10 minutes at 65 °C
• Synthesis of 2′Fluoro oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
• Consistent lot-to-lot high purity and performance
• Manufactured under a certified ISO 9001 quality system

Application

DMT-2′Fluoro-dG(ib) Phosphoramidite is suitable for use in the synthesis of 2′-fluoro-modified oligonucleotide sequences using automated solid-phase chemistry.

Molecular Weight: 857.91. Empirical Formula: C₄₄H₅₃FN₇O₈P. biological source: non-animal source (no BSE/TSE risk). Quality Level: 200. product line: Proligo Reagents. Assay: ≥. 99% (³¹P-NMR), ≥. 99.0% (reversed phase HPLC). form: powder. technique(s): oligo synthesis: suitable. impurities: ≤. 0.3% water content (Karl Fischer), ≤. 0.5% P(III) Impurities 100-169ppm (³¹P-NMR), ≤. 0.5% single Impurity (reversed phase HPLC), ≤. 3% residual Solvent content. color: white to off-white. λ: conforms (UV/VIS Identity). suitability: conforms to structure for H-NMR, conforms to structure for LC-MS. nucleoside profile: base: deoxyguanosine
base protecting group: isobutyryl
2' protecting group: fluoro
5' protecting group: DMT
deprotection: fast/standard. storage temp.: −. 20°C. SMILES string: COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](F)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(: O)NC(NC(: O)C(C)C): Nc34)(c5ccccc5)c6ccc(OC)cc6. InChI: 1S/C44H53FN7O8P/c1-27(2)40(53)49-43-48-39-37(41(54)50-43)47-26-51(39)42-36(45)38(60-61(58-24-12-23-46)52(28(3)4)29(5)6)35(59-42)25-57-44(30-13-10-9-11-14-30,31-15-19-33(55-7)20-16-31)32-17-21-34(56-8)22-18-32/h9-11,13-22,26-29,35-36,38,42H,12,24-25H2,1-8H3,(H2,48,49,50,53,54)/t35-,36-,38-,42-,61?/m1/s1. InChI key: KJFUMXZZVYMQEU-AOCJBPQJSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.