DCA DEBLOCK (TOLUENE) (C005B-225394)

Preparation Note

Toluene / Dichloroacetic acid 97/3 (v/v)

Other Notes

Deblock solution containing dichloroacetic acid (DCA) in toluene removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Quality Level: 200. type: for DNA synthesis, for DNA synthesis. product line: Proligo Reagents. storage condition: dry at room temperature. concentration: 42.2-51.6 . g/L (DCA content, titration). impurities: ≤. 200 . ppm water content (Karl Fischer). color: clear. compatibility: configured for ÄKTA^®. and OligoPilot^®. . Pictograms: GHS02,GHS05,GHS07,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H225 - H304 - H315 - H318 - H336 - H351 - H360FD - H373 - H410. Precautionary Statements: P210 - P273 - P280 - P301 + P310 - P305 + P351 + P338 - P331. Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 3 - Asp. Tox. 1 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Repr. 1B - Skin Irrit. 2 - STOT RE 2 - STOT SE 3. Target Organs: Central nervous system. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 39.2 °F. Flash Point(C): 4.0 °C.