Name: CHAPS HYDRATE BIOREAGENT SUITABLE FOR ELECTROPHORESIS =98% (HPLC) (C005B-033358)
SKU: C005B-033358
Availability: OutOfStock

CHAPS HYDRATE BIOREAGENT SUITABLE FOR ELECTROPHORESIS =98% (HPLC) (C005B-033358)

Catalog No.

C005B-033358

Manufacturer No.

C9426-1G

Manufacturer Name

Sigma-Aldrich

Quantity

Unit of Measure

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors.

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Description
Specifications

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

CHAPS has been used:

For membrane permeabilization of cells
As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
As a component in denaturing buffer in enzyme characterization
As a component of 2D- sample buffer in 2D electrophoresis
For protein solubilization in isoelectric focusing

Molecular Weight: 614.88 (anhydrous basis). Empirical Formula: C₃₂H₅₈N₂O₇S Â· xH₂O. description: zwitterionic. Quality Level: 200. product line: BioReagent. Assay: ≥. 98% (HPLC). form: powder. mol wt: 614.88 . g/mol (anhydrous basis). technique(s): electrophoresis: suitable, protein quantification: suitable. impurities: <. 0.04% DMF. mp: 157 . Â°C (dec.) (lit.). solubility: water: 50 . mg/mL, clear, colorless. suitability: suitable for electrophoresis. storage temp.: room temp. SMILES string: O.C[C@H](CCC(: O)NCCC[N+](C)(C)CCCS([O-])(: O): O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C. InChI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36. /h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41). 1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-. /m1./s1. InChI key: SJCUTFKCLFLIFE-JWTJKVBLSA-N. Pictograms: GHS07,GHS08. Signal Word: Danger. Hazard Statements: H302 - H360D. Precautionary Statements: P202 - P264 - P280 - P301 + P312 - P308 + P313 - P405. Hazard Classifications: Acute Tox. 4 Oral - Repr. 1B. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.