CAP A 2.5L AB SINGLE (C005B-225407)

General description

Cap A solution contains acetic anhydrideanhydride, lutidine and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step. The solution is configured for ABI.

Biochem/physiol Actions

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Tetrahydrofurane / Lutidine / Acetic Anhydride 8/1/1 (v/v/v)

type: for DNA synthesis. Quality Level: 200. product line: Proligo Reagents. shelf life: 365 . days. concentration: 97.0-119.0 . g/L (Acetic Anhydride content, titration). color: clear colorless, to brownish. compatibility: configured for ABI. storage temp.: 2-8°C. Pictograms: GHS02,GHS05,GHS06,GHS08. Signal Word: Danger. Hazard Statements: H225 - H302 - H315 - H318 - H331 - H335 - H336 - H351. Precautionary Statements: P210 - P280 - P301 + P312 - P304 + P340 + P311 - P305 + P351 + P338 - P308 + P313. Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2. Supp Hazards: EUH019. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 6.1 °F. Flash Point(C): -14.4 °C.