CAP A 2.5L AKTA SINGLE

General description

Cap A solution contains acetic anhydride and tetrahydrofuran (THF).

Application

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / N-Methylimidazole 8/2 (v/v)

Other Notes

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva