CAP 1 2.5L CAP1 2.5L

General description

Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).

Application

Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

type: for DNA synthesis. Quality Level: 200. product line: Proligo Reagents. storage condition: dry at room temperature. impurities: ≤. 200 . ppm water content (Karl Fischer). color: clear. suitability: conforms to structure (GC, Formulation). Pictograms: GHS02,GHS05,GHS07. Signal Word: Danger. Hazard Statements: H225 - H302 + H312 + H332 - H314. Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B. Storage Class Code: 3 - Flammable liquids. WGK: WGK 3. Flash Point(F): 35.6 °F - closed cup. Flash Point(C): 2.0 °C - closed cup.