BAFF ACTIVE HUMAN RECOMBINANT EXPRESSED IN NICOTIANA BENTHAMIANA

General description

BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.

Physical form

Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

Analysis Note

The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED₅₀ ≤ 50 ng/mL

Other Notes

Extinction Coefficient: E^0.1% = 0.791 (A₂₈₀ nm)
p.I: 6.03

biological source: human. Quality Level: 200. recombinant: expressed in Nicotiana. Assay: >. 97% (SDS-PAGE). form: lyophilized. potency: ≤. 50 . ng/mL ED₅₀. mol wt: 18-20 . kDa (a glycosilated polypeptide chain containing 151 amino acids). packaging: pkg of 10 . μ. g. storage condition: avoid repeated freeze/thaw cycles. impurities: Endotoxin, tested (LAL test, <. 0.04 EU/ ug protein). UniProt accession no.: Q9Y275. storage temp.: −. 20°C. Gene Information: human ... TNFSF13B(10673). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.