AZURIN FROM PSEUDOMONAS AERUGINOSA

Application

Azurin has been used:

• in the cytotoxicity and cell viability studies in human osteosarcoma cell line
• for the functionalization of silicon nitride cantilevers for interaction studies
• for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica

Biochem/physiol Actions

Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.

Physical form

Lyophilized powder containing ammonium acetate buffer salts.

biological source: Pseudomonas aeruginosa. Quality Level: 200. form: lyophilized powder. composition: Protein, ≥. 65% Lowry . concentration: ≥. 65.0% (Lowry). technique(s): toxicology assay: suitable. UniProt accession no.: P00282. storage temp.: −. 20°C. Gene Information: Pseudomonas aeruginosa ... AZU(878046). Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).