AZURE A CERTIFIED (C.I. 52005) (C005B-046905)

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.

Biochem/physiol Actions

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Suitability

Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Molecular Weight: 291.80. Empirical Formula: C₁₄H₁₄ClN₃S. grade: certified by the Biological Stain Commission. Quality Level: 200. form: powder. composition: Dye content, 70% . mp: 290 . °C (dec.) (lit.). solubility: H₂O: 1 . mg/mL. λ. _max: 633 nm. ε (extinction coefficient): ≥. 11000 at 242-246 . nm in H₂O at -1, ≥. 26000 at 287-291 . nm in H₂O, ≥. 34000 at 620-634 . μ. m in H₂O. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: room temp. SMILES string: NC1: CC(SC(C2: N3): CC(C: C2): [N+](C)C): C3C: C1.[Cl-]. InChI: 1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12. /h3-8,15H,1-2H3. 1H. InChI key: NALREUIWICQLPS-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).