EOSIN B CERTIFIED (C.I. 45400) (C005B-048145)

Application

• Eosin B has been used for the histological analysis of anal sphincter sections obtained from rats.
• It has been used along with Mayer′s hematoxylin for the staining of triangularis sterni muscle sections.
• It has been used along with hematoxylin for the staining of different tissue sections obtained from mouse.

Biochem/physiol Actions

Eosin B is a xanthene dye and is also referred to as eosin bluish or erythrosin B. It is used in the Harris stain for Negri bodies. Eosin B is also used in Mayer′s Hemalum as a counterstain for collagen. Eosin B-Azure A are used together for the staining of cell granules, nuclei and microorganisms. It can also be used for protein estimation. The protein - eosin B complex absorbs at wavelength range of 536–544nm and the change in blue shift helps in determining the protein concentration.

Suitability

Counterstain for collagen, following Mayer′s Hemalum. Used with Azure A as a tissue stain for cell granules, nuclei, and microorganisms. For differential staining of cells of the anterior pituitary.

Molecular Weight: 624.06. Empirical Formula: C₂₀H₆Br₂N₂Na₂O₉. linearFormula: C₂₀H₆N₂O₉Br₂Na₂. grade: certified by the Biological Stain Commission. Quality Level: 200. form: powder. composition: Dye content, 90% . solubility: H₂O: 1 . mg/mL, clear, red. λ. _max: 395 nm, 514 nm. application(s): diagnostic assay manufacturing
hematology
histology. storage temp.: room temp. SMILES string: [Na+].[Na+].[O-]C(: O)c1ccccc1C2: C3C: C(C(: O)C(Br): C3Oc4c(Br)c([O-])c(cc24)[N+]([O-]): O)[N+]([O-]): O. InChI: 1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14. . /h1-6,25H,(H,27,28). . /q. 2*+1/p-2. InChI key: GYYTYUGGVBYJHE-UHFFFAOYSA-L. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).