APN-CHO

DescriptionAPN-Aldehyde is a bifunctional crosslinker for introduction of aldehyde into biomolecules. The coupling can be performed with high selectivity in biological medium using mild reaction conditions.Preparation instructionsStandard protein labeling procedure (cysteine labeling).Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).Incubate at room temperature for 2 hours.If necessary, purify the protein-aldehyde conjugate using size exclusion chromatography or ultrafiltration.The conjugate can be readily coupled with hydrazides/hydrazones/hydroxylamines using standard protocols.

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