ANTI-AQUAPORIN 5 RABBIT PAB

General description

Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.

Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.

Immunogen

a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5

Application

ELISA (0.5-1.0 µg/ml)

Immunoblotting (1-10 µg/ml)

Immunofluorescence (2-20 µg/ml)

Immunohistochemistry (2-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Epithelial cells

Other Notes

Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.

Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity: rat, mouse, human. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.