ANTI-VASOPRESSIN RABBIT PAB

General description

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.

Recognizes vasopressin in rat hypothalamus.

This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.

Immunogen

Rat

rat arginine vasopressin

Application

Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.

Analysis Note

Positive Control
Rat hypothalamus

Other Notes

Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.

Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany