Application
Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 . Product A2714 is essentially sulfate-free and contains citrate buffer salts.
Biochem/physiol Actions
Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold .
Unit Definition
One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.
Physical form
Essentially sulfate-free containing citrate buffer salts
biological source: rabbit muscle. Quality Level: 300. form: lyophilized powder. specific activity: ≥. 8.0 . units/mg protein. composition: Protein, ≥. 80% biuret . foreign activity: glyceraldehyde-3-phosphate dehydrogenase ≤. 0.03%, lactic dehydrogenase ≤. 0.03%, phosphoglucose isomerase ≤. 0.6%, pyruvate kinase ≤. 0.1%, triosephosphate isomerase ≤. 0.05%. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).- UPC:
- 51151904
- Condition:
- New
- HazmatClass:
- No
- MPN:
- A2714-200UN
- CAS:
- 9024-52-6
akash.verma@cenmed.com
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