ALDOLASE TYPE IV FROM RABBIT MUSCLE AMMONIUM SULFATE (C005B-088958)

General description

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.

Application

Aldolase from rabbit muscle has been used:

• in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay
• as a standard in the characterization of metabolic enzymes from glaucomatous tissues
• in fructose 2,6-bisphosphate assay of human cell lines

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 .

Biochem/physiol Actions

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics. It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold .

Unit Definition

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Physical form

Crystalline suspension in 2.5 M (NH₄)₂SO₄, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Analysis Note

Protein determined by biuret.

biological source: rabbit muscle. Quality Level: 200. form: ammonium sulfate suspension. specific activity: 10-20 . units/mg protein. foreign activity: glyceraldehyde-3-phosphate dehydrogenase ≤. 0.03%, lactic dehydrogenase ≤. 0.03%, phosphoglucose isomerase ≤. 0.6%, pyruvate kinase ≤. 0.1%, triosephosphate isomerase ≤. 0.05%. storage temp.: 2-8°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.