YEAST LYSIS BUFFER. 500ML

Yeast PE LBTM is useful for extraction of soluble proteins from yeast cells. Yeast PE LBTM is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast PE LBTM is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided.Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast- PE LBTM (see Related Products for protease inhibitor Protease ArrestTM). The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast PE LBTM eliminates the need for laborious glass bead lysis of yeast cells. The kit is good for 100 preps of 50μl yeast cell pellet. APPLICATIONS Preparation of yeast spheroplast and extraction of yeast proteins. This kit is suitable for processing approximately 10ml yeast cell pellet suspension, either single or multiple smaller preps. COMPATIBILITY Yeast PE LBTM is compatible with any downstream application including running various chromatography procedures and gel electrophoresis applications. Yeast PE LBTM is also compatible for protein estimation with NITM protein assay . Features Eliminates the need for glass bead lysis. Supplied as a kit, containing Zymolyase® and reagents needed for protein extraction. Suitable for processing 100 x 50μl yeast cell pellet preparations. Applications Lysis and extraction of proteins from yeast cells. Isolation of spheroplasts. For the isolation of biologically active proteins. Suitable for most applications including enzyme and protein purifications, electrophoresis, Western blotting and 2D-gel analysis.