WILKINS CHALGREN ANAEROBIC AGAR

Application

Used for the isolation of anaerobic bacteria.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 5.00
Dextrose, 1.00
Sodium chloride, 5.00
L-Arginine, 1.00
Sodium pyruvate, 1.00
Hemin, 0.005
Menadione, 0.0005
Agar, 10.00

Preparation Note

Suspend 43 g of Wilkins Chalgren Anaerobic Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates.For the cultivation of anaerobes add 2 vial of Non-Spore Anaerobic Supplement (Cat. No. N8781) or 2 vial of G.N. Spore Anaerobic Supplement (Cat. No. G3789) as desired into the sterile molten medium before pouring into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany