Western Rapid Transfer Buffer (10X, Wet & Semi-dry) (C007B-566660)

The Transfer Buffer turned light brown or yellow-brown should be discarded.When stored at 4℃ for a long period of time, precipitation might occur in this product. The precipitates must be dissolved completely before use.Analytical grade or high-purity ethanol is recommended for preparation of transfer buffer.If the current or voltage during the transfer is high, the generated Joule heating leads to a lower resistance of the transfer buffer, which ultimately affects the transfer efficiency, the buffering capacity of the transfer buffer, and even causes the gel to melt or adhere to the membrane. Therefore, we recommend performing the transfer in an ice box or at 4℃..When using this product for protein transfer and setting the constant current at 400mA, the voltage is generally 150-200V at the beginning no matter there is 1 or 2 pieces of membrane in the transfer tank. As the temperature of the transfer buffer will gradually increase and the resistance will become smaller, the voltage is generally 100-150V at the end. The transfer efficiency is related to the electric field strength (E) which is mainly related to the voltage and the distance between the electrodes, i.e., E=U/d.We recommend using Power™ Elite Power Supply (300V/600mA/100W) or Power™ Prime Power Supply (300V/2000mA/200W) for Western transfer.The temperature of the transfer buffer during the transfer, the parameters of the gel (e.g., number, thickness, ionic concentration, acrylamide concentration, etc.), the thickness of the filter paper, the number of transfer tanks used simultaneously, the ionic concentration of the transfer buffer, the volume of the transfer buffer, and the number of times the transfer buffer is used may all affect the current and voltage. Among them, the number of simultaneous transfer tanks used for one electrophoresis is the most critical factor. In case of constant current, each additional transfer tank will reduce the current and voltage of each tank accordingly. If 2 or more transfer tanks need to be used at the same time, it is recommended to use a high power supply with high current, or set a lower current or voltage for a longer transfer time.When transferring, if an error is reported, check whether there is an overload of voltage or current, or abnormal conditions such as too much or too little transfer buffer, short circuit or breakage of the transfer tank, etc..This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. Preparation of Western Rapid Transfer Buffer.a. Add 100ml of Western Rapid Transfer buffer (10X) into 700ml of distilled, ultrapure or deionized water.b. Add 200ml of absolute ethanol or 210ml of 95% ethanol.c. Make up to a final volume of 1 liter with distilled, ultrapure or deionized water, and mix well.Note: Western Rapid Transfer buffer pre-cooled at 4℃ before use is more effective. New Rapid Transfer buffer can be stored at room temperature or 4℃. The Transfer Buffer turned light brown or yellow-brown should be discarded.2. Transfer conditions: Protein transfer can be performed at 500mA constant current for 15-20min (external ice bath recommended) or at 400mA constant current for 20-25min (with an internal ice box only; using an external ice bath is more effective). Appropriate reduce the transfer time for small proteins and increase the transfer time for bigger proteins.3. For detailed Western operations, please refer to http://www.aladdin-e.com/support/western.htm.1. Preparation of Western Rapid Transfer Buffer.a. Add 100ml of Western Rapid Transfer buffer (10X) into 700ml of distilled, ultrapure or deionized water.b. Add 200ml of absolute ethanol or 210ml of 95% ethanol.c. Make up to a final volume of 1 liter with distilled, ultrapure or deionized water, and mix well.Note: Western Rapid Transfer buffer pre-cooled at 4℃ before use is more effective. New Rapid Transfer buffer can be stored at room temperature or 4℃. The Transfer Buffer turned light brown or yellow-brown should be discarded.2. Transfer conditions: Protein transfer can be performed at 500mA constant current for 15-20min (external ice bath recommended) or at 400mA constant current for 20-25min (with an internal ice box only; using an external ice bath is more effective). Appropriate reduce the transfer time for small proteins and increase the transfer time for bigger proteins.3. For detailed Western operations, please refer to http://www.aladdin-e.com/support/western.htm.Related Products:产品编号产品名称包装E6001MiniProGel™ Protein Gel-Cast and Electrophoresis System (4-gel)1SetE6005MiniProGel™ Protein Gel-Cast and Electrophoresis System (2-gel)1SetE6050MiniBlot™ Electrophorectic Transfer Cell1SetE6053 MiniBlot™ Electrophorectic Transfer Central Core1SetE6080 Elite Power Supply (300V/600mA/100W)1SetE6085 Prime Power Supply (300V/2000mA/200W)1SetP0021AWestern Transfer Buffer1LP0021BWestern Transfer Buffer10×1LP0572-100mlWestern Rapid Transfer Buffer (10X)100mlP0572-2LWestern Rapid Transfer Buffer (10X)4×500mlP0572-500mlWestern Rapid Transfer Buffer (10X)500mlP0575-10LWestern Rapid Transfer Buffer (Powder)10×1LP0575-1LWestern Rapid Transfer Buffer (Powder)1L

Specification: 10X