WEE1 ACTIVE 10UG

General description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ′Tyr-15′. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on ′Tyr-15′ and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Product Source: N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.

Biochem/physiol Actions

Protein Target: WEE1

Packaging

Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.

Quality

Routinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: human. Quality Level: 100, 300. recombinant: expressed in baculovirus infected Sf21 cells. mol wt: M_w 77 . kDa. manufacturer/tradename: Upstate^®. . technique(s): activity assay: suitable (kinase). NCBI accession no.: NM_003390. UniProt accession no.: P30291. Gene Information: human ... WEE1(7465). Pictograms: GHS07. Signal Word: Warning. Hazard Statements: H317. Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364. Hazard Classifications: Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.