General description
Molecular biology grade water is sterile filtered and ready to use, free from DNase, RNase, phosphatase, and protease contamination.
Application
Water, sterile filtered has been used:
- for cloning and plasmid construction.
- for restriction digestion of PCR amplicons in polysaccharide A (PSA) restriction method.
- in RNA isolation.
- fluorescence in situ hybridization (FISH).
- to dilute the samples of CVF (cervicovaginal fluid) and menstrual extracts for testing specificity through reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of mRNA.
- for DNA quantification using quantitative polymerase chain reaction (qPCR).
Molecular Weight: 18.02. Empirical Formula: H
2O. linearFormula: H
2O. grade: for molecular biology. Quality Level: 100. sterility: sterile-filtered. form: liquid. quality: sterile filtered. shelf life: limited shelf life, expiry date on the label. technique(s): FISH: suitable. impurities: DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected. refractive index:
n20/D 1.34 (lit.). pH: 5-7. conductivity: 0.0-5 . μ. S/cm at 25 . °C. bp: 100 . °C (lit.). mp: 0 . °C (lit.). density: 1.000 . g/mL . at 3.98 . °C (lit.). cation traces: Ca: <. 10 . ppb, Mg: <. 10 . ppb. SMILES string: O. InChI: 1S/H2O/h1H2. InChI key: XLYOFNOQVPJJNP-UHFFFAOYSA-N. Storage Class Code: 10 - Combustible liquids. WGK: nwg. Flash Point(F): No data available. Flash Point(C): No data available. Personal Protective Equipment: Eyeshields, Gloves.