VITRONECTIN HUMAN

General description

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. Recombinant humanVitronectin is a protein consisting of 459 amino acids. It exists in two forms:a single chain and a clipped form consisting of two chains held together bydisulfide bonds resulting from an endogenous cleavage. Under reducingconditions, the single chain migrates at an apparent molecular mass of 75kDa onSDS-PAGE, and the clipped form migrates at molecular masses of 65kDa and 10kDa.Recombinant vitronectin can function as a chemically defined matrix componentin human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Biochem/physiol Actions

The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin α_vβ₃ enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. Vitronectin is involved in wound repair and also controls complement system. It works as a marker of profibrotic activity in liver, heart and kidney.

Sequence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Physical form

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.