VEGF-B HUMAN

General description

VEGFB (vascular endothelial growth factor B) is one of the seven members of the VEGF family, which also includes VEGF-A, VEGF-C, VEGF-D, VEGF-E, VEGF-F, and PIGF (placental growth factor). All these members contain a common VEGF homology domain. This domain forms the core region and contains a cystine knot motif composed of eight invariant cysteine residues. VEGFB, discovered in 1995, is highly expressed in adult myocardium, skeletal muscle, and pancreas. This gene contains seven exons and alternative splicing results in the synthesis of two isoforms VEGF-B167 of 21 kDa and VEGF-B186 of 32 kDa. These isoforms show differential level of expression with VEGF-B167 being the predominantly expressed isoform. VEGFB gene is localized to human chromosome 11q13.
Recombinant human VEGF-B is a 38.0 kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.

Biochem/physiol Actions

VEGF-B, a member of the VEGF family, is a potent growth and angiogenic cytokine. Recombinant human VEGF-B is a 38.0 kDa disulfide-linked homodimeric protein consisting of two 167 amino acid polypeptide chains.

VEGFB (vascular endothelial growth factor B) acts on the receptors VEGFR-1 (VEGF receptor) and NRP-1 (neuropilin). This protein induces coronary vessel growth and cardiac hypertrophy, which might confer protection to heart against ischemic damage and heart failure. It might have a role in metabolic functions as it shows high expression levels in tissues with highly active energy metabolism. VEGFB is also thought to be involved in neuroprotection. This protein is involved in fetal angiogenesis, functions as a mitogen for endothelial cells (ECs), survival factor for multiple types of cells such as neurons, vascular cells, and myocytes.

Sequence

PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR

Physical form

Lyophilized from 10 mM Acetic Acid.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.