VASCULAR ENDOTHELIAL GROWTH FACTOR (AA 207-318) HUMAN RECOMBINANT EXPRESSED IN ESCHERICHIA COLI

Application

Vascular Endothelial Growth Factor (aa 207-318) human also known as VEGF 121 was used for preparing a cellular matrix hybridized with hyaluronic acid (ACM-HA) VEGF constructs which is used in measuring the VEGF-enhanced tissue-engineered bladder graft neovascularization by using dynamic contrast-enhanced MRI. The product was used for creating VEGF-impregnated tissue-engineered construct which was inserted in the critical-sized calvarial defect in the membranous parietal bone of the rabbit for examining and characterizing the angiogenesis necessary for bone formation.

Biochem/physiol Actions

Vascular Endothelial Growth Factor (aa 207-318) human also referred to as VEGF 121 or Vasculotropin is a basic protein that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF also enhances the growth of endothelial cells isolated from cerebral cortex, fetal and adult aorta as well as human umbilical vein. Cells enhance VEGF 121 secretion from fibrin implants results in local and controlled blood vessel growth.

Potent angiogenesis inducer in vivo.

Other Notes

112 amino acid residue variant of human VEGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetronitrile and 0.1% TFA containing 0.25 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in human umbilical vein endothelial cells.