UREA EXTRA PURE DAB PH. EUR. B. P. U (C005B-049551)

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.
Urea can be prepared from ammonia and carbon-dioxide, via Haber-Bosch process. It can also be used to prepare urea-formaldehyde resins, via condensation reaction.

Application

Urea has been used in the following studies:

• Synthesis of SnO₂ nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain
• Protein electrophoretic studies.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Molecular Weight: 60.06. Empirical Formula: CH₄N₂O. linearFormula: NH₂CONH₂. grade: puriss.. Quality Level: 200. Assay: 99.0-100.5%, 99.0-101.0% (calc. on dry substance). form: solid. quality: meets analytical specification of Ph. Eur., BP, USP. impurities: alkalic reac. substances, complies, ≤. 0.001% heavy metals (as Pb), ≤. 0.04% insoluble in ethanol, ≤. 0.05% ammonium (NH₄), ≤. 0.1% biuret(carbamylurea). ign. residue: ≤. 0.1% (as SO₄). loss: ≤. 1.0% loss on drying, 105 °C, 1 h. mp: 132-135 . °C (lit.), 132-135 . °C. solubility: H₂O: soluble 480 . g/L at . 20 . °C. density: 1.335 . g/mL . at 25 . °C (lit.). anion traces: chloride (Cl^-): ≤. 10 . mg/kg, sulfate (SO₄^2-): ≤. 50 . mg/kg. cation traces: Fe: ≤. 0.5 . mg/kg. suitability: in accordance for appearance of solution, in accordance for identity. SMILES string: NC(N): O. InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4). InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).