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ubiquitin e1 enzyme (ube1), human recombinant

Ubiquitin E1 Enzyme (UBE1), human recombinant

Catalog No.
C007B-440691
Manufacturer No.
U388907-50μl
Manufacturer Name
Aladdin Scientific
Quantity
1
Unit of Measure
EA

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This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and

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This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.

Specifications and Purity: 0.5 μg/μl in PBS containing 0.01% sodium azide

EC Number: 230-907-9

Related Documents: https://aladdin-for-icloud-store.oss-cn-hangzhou.aliyuncs.com/aladdinsci/pdp/sds/1/U388907-SCI_7802e6a1e63a36d56c3f42b67c1b8ee2.pdf

UPC:
41106618
Condition:
New
HazmatClass:
No
WeightUOM:
LB
MPN:
U388907-50μl
Product Size:
50μl
Precautionary Statement Codes:
P264

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