TWISTED GASTRULATION PROTEIN HUMAN

General description

Twisted gastrulation protein (TSG) is a secreted bone morphogenetic protein (BMP)-binding protein structurally related to the BMP antagonists chordin and noggin. The gene encoding TSG is localized on human chromosome 18p11.22. Recombinant human TSG is a 199 amino acid 22.2kDa protein containing the BMP/TGFb binding portion of the full length TSG protein.

Biochem/physiol Actions

Twisted gastrulation protein (TSG) can inhibit bone morphogenetic protein (BMP) activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/chordin complex.

Sequence

MCNKALCASD VSKCLIQELC QCRPGEGNCS CCKECMLCLG ALWDECCDCV GMCNPRNYSD TPPTSKSTVE ELHEPIPSLF RALTEGDTQL NWNIVSFPVA EELSHHENLV SFLETVNQPH HQNVSVPSNN VHAPYSSDKE HMCTVVYFDD CMSIHQCKIS CESMGASKYR WFHNACCECI GPECIDYGSK TVKCMNCMF

Physical form

Lyophilized from 10 mM Sodium Acetate, pH 6.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.