Trypsin from bovine pancreas (C007B-566503)

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestions. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps. Trypsin from bovine pancreas has been used:-For Trypsinization, for eliminating the membrane bound alloantigen.For incubating 20-kD amylase-binding component, to evaluate its Chemical nature.To prepare lactoferrin degradation products for evaluating its antiadipogenic activity.

Specification: Type XI, lyophilized powder,≥6,000 BAEE units/mg protein

MDL Number: MFCD00082094

EC Number: 232-650-8