TRYPSIN FROM BOVINE PANCREAS TPCK TREATED ON BEADED AGAROSE (C005B-287609)

Catalog No.: C005B-287609
Manufacturer No.: T4019-25UN
Manufacturer Name: Sigma-Aldrich
Quantity: 25
Unit of Measure: UN

General description The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues: the other is related to the 8-anilinonaphthalene-1-sulfonate binding. Application An experimental approach using

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Description
Specifications

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Application

An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.

Unit Definition

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 Â°C (titrimetric assay).

Physical form

Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative

Preparation Note

TPCK-treated

UPC:: 51131508
Condition:: New
HazmatClass:: No
MPN:: T4019-25UN
Temperature Control Device:: Yes

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